Journal of Leukocyte Biology, Vol 55, Issue 6 729-734, Copyright © 1994 by Society for Leukocyte Biology
JOURNAL ARTICLE |
G Bouvier, AM Benoliel, C Foa and P Bongrand
INSERM U 387, Laboratoire d'Immunologie, Hopital de Sainte-Marguerite, Marseille, France.
The fate of pathogens ingested by macrophages is dependent on phagosome acidification and fusion with different intracellular vesicles. Whereas the mode of particle recognition by the phagocyte seems the main determinant of phagosome-lysosome fusion, the influence of membrane reorganization, fusion events, and cell activation in phagosome acidification is not well known. We looked for a relationship between the nature of receptors involved in phagocytosis, phagosome acidification, and phagosome-lysosome fusion. Murine macrophage-like P388D1 cells were made to ingest sheep erythrocytes coated with immunoglobulin G (EIgG) or IgM and complement (EIgMC) or treated with glutaraldehyde and periodate (EGP). The following results were obtained: (1) As expected, the adhesion of the three particle types was differentially inhibited by monoclonal antibodies specific for Fc gamma RII and CD11b/CD18. (2) The phagosomes containing all three particle types displayed similar acidification kinetics with a pH decrease to 6 within the first 10 min after ingestion. (3) Only phagosomes containing EIgG or EIgMC were fused with peroxidase-loaded secondary lysosomes. (4) Coating EGP with IgG only partially restored fusion, even when the surface density of IgG was markedly higher than found on EIgG. It is concluded that phagosome acidification and fusion are regulated by different mechanisms. Also, the lack of fusion observed with EGP is not entirely accounted for by the absence of stimulation of suitable receptors on the phagocyte membrane, because it cannot be restored by providing such a stimulus.
This article has been cited by other articles:
 |
|
 |
|
  H. Mehta, M. Glogauer, S. Becart, A. Altman, and K. M. Coggeshall Adaptor Protein SLAT Modulates Fc{gamma} Receptor-mediated Phagocytosis in Murine Macrophages J. Biol. Chem., May 1, 2009; 284(18): 11882 - 11891. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Kang, A. K. Azad, J. B. Torrelles, T. M. Kaufman, A. Beharka, E. Tibesar, L. E. DesJardin, and L. S. Schlesinger The human macrophage mannose receptor directs Mycobacterium tuberculosis lipoarabinomannan-mediated phagosome biogenesis J. Exp. Med., October 3, 2005; 202(7): 987 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bauer and H. Tapper Membrane retrieval in neutrophils during phagocytosis: inhibition by M protein-expressing S. pyogenes bacteria J. Leukoc. Biol., December 1, 2004; 76(6): 1142 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Tempone, D. Perez, S. Rath, A. L. Vilarinho, R. A. Mortara, and H. F. de Andrade Jr Targeting Leishmania (L.) chagasi amastigotes through macrophage scavenger receptors: the use of drugs entrapped in liposomes containing phosphatidylserine J. Antimicrob. Chemother., July 1, 2004; 54(1): 60 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Astarie-Dequeker, S. Carreno, C. Cougoule, and I. Maridonneau-Parini The protein tyrosine kinase Hck is located on lysosomal vesicles that are physically and functionally distinct from CD63-positive lysosomes in human macrophages J. Cell Sci., January 1, 2002; 115(1): 81 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Rittig, M.-T. Alvarez-Martinez, F. Porte, J.-P. Liautard, and B. Rouot Intracellular Survival of Brucella spp. in Human Monocytes Involves Conventional Uptake but Special Phagosomes Infect. Immun., June 1, 2001; 69(6): 3995 - 4006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Porte, J.-P. Liautard, and S. Kohler Early Acidification of Phagosomes Containing Brucella suis Is Essential for Intracellular Survival in Murine Macrophages Infect. Immun., August 1, 1999; 67(8): 4041 - 4047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Strasser, S. L. Newman, G. M. Ciraolo, R. E. Morris, M. L. Howell, and G. E. Dean Regulation of the Macrophage Vacuolar ATPase and Phagosome-Lysosome Fusion by Histoplasma capsulatum J. Immunol., May 15, 1999; 162(10): 6148 - 6154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Hackam, O. D. Rotstein, W.-J. Zhang, N. Demaurex, M. Woodside, O. Tsai, and S. Grinstein Regulation of Phagosomal Acidification. DIFFERENTIAL TARGETING OF Na+/H+ EXCHANGERS, Na+/K+-ATPases, AND VACUOLAR-TYPE H+-ATPases J. Biol. Chem., November 21, 1997; 272(47): 29810 - 29820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Hackam, O. D. Rotstein, A. Schreiber, W.-j. Zhang, and S. Grinstein Rho is Required for the Initiation of Calcium Signaling and Phagocytosis by Fc{gamma} Receptors in Macrophages J. Exp. Med., September 15, 1997; 186(6): 955 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Alvarez-Dominguez, R Roberts, and P. Stahl Internalized Listeria monocytogenes modulates intracellular trafficking and delays maturation of the phagosome J. Cell Sci., January 3, 1997; 110(6): 731 - 743. [Abstract] [PDF]
|
|
|
|
|
|
H. Collins, U. Schaible, J. Ernst, and D. Russell Transfer of phagocytosed particles to the parasitophorous vacuole of Leishmania mexicana is a transient phenomenon preceding the acquisition of annexin I by the phagosome J. Cell Sci., January 1, 1997; 110(2): 191 - 200. [Abstract] [PDF]
|
|
|
|
|
|
C. Alvarez-Dominguez, A. M. Barbieri, W. Beron, A. Wandinger-Ness, and P. D. Stahl Phagocytosed Live Listeria monocytogenes Influences Rab5-regulated in Vitro Phagosome-Endosome Fusion J. Biol. Chem., June 7, 1996; 271(23): 13834 - 13843. [Abstract] [Full Text] [PDF]
|
|
