Accuri C6 Flow Cytometer System

This Article
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gardner, C. R.
Right arrow Articles by Laskin, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gardner, C. R.
Right arrow Articles by Laskin, D. L.

Journal of Leukocyte Biology, Vol 53, Issue 2 190-196, Copyright © 1993 by Society for Leukocyte Biology

JOURNAL ARTICLE

Platelet-activating factor-induced calcium mobilization and oxidative metabolism in hepatic macrophages and endothelial cells

CR Gardner, JD Laskin and DL Laskin
Department of Pharmacology, Rutgers University, Piscataway, NJ 08855-0789.

Fluorescence image analysis of the calcium sensitive dye Indo-1 was used to characterize platelet-activating factor (PAF)-induced calcium mobilization in hepatic macrophages and endothelial cells. PAF, but not lyso-PAF, an inactive analog, induced a rapid and transient increase in intracellular levels of calcium that appeared to depend on the presence of extracellular calcium. In both macrophages and endothelial cells, these effects were dose dependent, reaching maximal levels with 10 nM PAF. However, the kinetics of the calcium response to PAF in macrophages and endothelial cells was distinct. The endothelial cells responded more rapidly to PAF than the macrophages (within 1 min vs. 2-3 min, respectively) and displayed longer recovery periods (4 min vs. > 10 min, respectively). In contrast, the magnitude of the response to PAF was greater in the macrophages. In both cell types, triazolam and alprazolam, two PAF antagonists, and the calcium channel blocker verapamil inhibited PAF-induced calcium mobilization. PAF also stimulated superoxide anion production alone and in combination with the macrophage activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) in both cell types. Macrophages produced more of this reactive oxygen intermediate in response to PAF and TPA than endothelial cells. Interestingly, in the absence of extracellular calcium or when verapamil was added to the cultures, superoxide anion production by the macrophages, but not the endothelial cells, was significantly reduced. These results demonstrate that, although hepatic macrophages and endothelial cells mobilize calcium in response to PAF, the characteristics of the response in each cell type are different. These differences may underlie, at least in part, distinct functional responses of the cells to PAF.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Q. Hu, Z.-X. Yu, V. J. Ferrans, K. Takeda, K. Irani, and R. C. Ziegelstein
Critical Role of NADPH Oxidase-derived Reactive Oxygen Species in Generating Ca2+ Oscillations in Human Aortic Endothelial Cells Stimulated by Histamine
J. Biol. Chem., August 30, 2002; 277(36): 32546 - 32551.
[Abstract] [Full Text] [PDF]

Home page
 Arch SurgHome page
C.-J. Lo, H. G. Cryer, M. Fu, and B. Kim
Endotoxin-Induced Macrophage Gene Expression Depends on Platelet-Activating Factor
Arch Surg, December 1, 1997; 132(12): 1342 - 1347.
[Abstract] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. Chen and S. N. Giri

J. Pharmacol. Exp. Ther., June 1, 1997; 281(3): 1047 - 1058.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
D Ohmer-Schrock, C Schlatterer, H Plattner, and J Schlepper-Schafer
Lung surfactant protein A (SP-A) activates a phosphoinositide/calcium signaling pathway in alveolar macrophages
J. Cell Sci., January 12, 1995; 108(12): 3695 - 3702.
[Abstract] [PDF]