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Journal of Leukocyte Biology, Vol 50, Issue 5 471-478, Copyright © 1991 by Society for Leukocyte Biology


JOURNAL ARTICLE

Murine eosinophil granulocytes bind the murine macrophage-monocyte specific monoclonal antibody F4/80

MP McGarry and CC Stewart
Department of Laboratory Animal Resources, Roswell Park Cancer Institute, Buffalo, New York 14263.

Studies designed to identify a panel of monoclonal antibodies useful for the separation of murine eosinophil myeloid progenitors revealed that F4/80, an antigen heretofore thought to be expressed only by murine monocyte/macrophage lineage cells, was expressed by eosinophil granulocytes. Eosinophils from several strains of mice stained positively with specific antibody for the epitope. A novel pathway for myeloid differentiation is proposed in which neutrophil progenitors exit a common lineage prior to a common progenitor of monocytes and eosinophils. Moreover, the results demonstrate that binding of anti-F4/80 can no longer be viewed as exclusive for mononuclear phagocytes.


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