Journal of Leukocyte Biology, Vol 49, Issue 6 579-586, Copyright © 1991 by Society for Leukocyte Biology
JOURNAL ARTICLE |
AJ Pinto, D Stewart, N van Rooijen and PS Morahan
Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
The current results provide direct evidence for a role of tissue macrophages (M phi) in natural immunity and support the use of immunomodulators to enhance antiviral resistance in immunocompromised individuals. In this study, macrophages (M phi) in the spleen and liver were eliminated by intravenous (i.v.) injection of the drug dichloromethylene diphosphonate (DMDP) encapsulated in liposomes. The effect of this depletion system on peritoneal M phi, peripheral blood leukocytes, splenic natural killer (NK) activity, and natural and immunomodulator-induced host resistance was then assessed. Barrier-maintained CD-1 female mice were inoculated i.v. either with DMDP liposomes, free liposomes (containing no DMDP), or saline on day -2 or on days -3 and -1 before cell population analysis or infection. Single or double treatment with DMDP liposomes had no effect on peritoneal M phi as indicated by no changes in total number, differential counts, or ectoenzyme patterns. Double treatment with DMDP liposomes caused a marked leukocytosis in blood, primarily of lymphocytes and polymorphonuclear leukocytes (PMN), and a transient depression of spontaneous and interferon-inducible splenic NK activity. The effects on host resistance to i.v. infection with Listeria monocytogenes or herpes simplex virus type 2 (HSV-2) indicated that i.v. treatment with DMDP liposomes significantly reduced natural resistance to these microorganisms as evidenced by increased mortality and decreased median survival time. When DMDP liposomes-treated mice were given the immunomodulator maleic anhydride divinyl ether copolymer (MVE-2) intraperitoneally the day before infection with HSV-2, the immunosuppressive effect of DMDP liposomes treatment was significantly reversed.
This article has been cited by other articles:
 |
|
 |
|
  A. Mildner, M. Djukic, D. Garbe, A. Wellmer, W. A. Kuziel, M. Mack, R. Nau, and M. Prinz Ly-6G+CCR2- Myeloid Cells Rather Than Ly-6ChighCCR2+ Monocytes Are Required for the Control of Bacterial Infection in the Central Nervous System J. Immunol., August 15, 2008; 181(4): 2713 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lombry, D. A. Edwards, V. Preat, and R. Vanbever Alveolar macrophages are a primary barrier to pulmonary absorption of macromolecules Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1002 - L1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Zhonghua Li and C. M. Blatteis Fever onset is linked to the appearance of lipopolysaccharide in the liver Innate Immunity, February 1, 2004; 10(1): 39 - 53. [Abstract] [PDF]
|
|
|
|
|
|
P. Aichele, J. Zinke, L. Grode, R. A. Schwendener, S. H. E. Kaufmann, and P. Seiler Macrophages of the Splenic Marginal Zone Are Essential for Trapping of Blood-Borne Particulate Antigen but Dispensable for Induction of Specific T Cell Responses J. Immunol., August 1, 2003; 171(3): 1148 - 1155. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Jordan, N. van Rooijen, S. Izui, J. Kappler, and P. Marrack Liposomal clodronate as a novel agent for treating autoimmune hemolytic anemia in a mouse model Blood, January 15, 2003; 101(2): 594 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Gregory and E. J. Wing Neutrophil-Kupffer cell interaction: a critical component of host defenses to systemic bacterial infections J. Leukoc. Biol., August 1, 2002; 72(2): 239 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Gregory, L. P. Cousens, N. van Rooijen, E. A. Dopp, T. M. Carlos, and E. J. Wing Complementary Adhesion Molecules Promote Neutrophil- Kupffer Cell Interaction and the Elimination of Bacteria Taken Up by the Liver J. Immunol., January 1, 2002; 168(1): 308 - 315. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Guleria and J. W. Pollard Aberrant Macrophage and Neutrophil Population Dynamics and Impaired Th1 Response to Listeria monocytogenes in Colony-Stimulating Factor 1-Deficient Mice Infect. Immun., March 1, 2001; 69(3): 1795 - 1807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Thomas, Y. Li, T. Kodama, H. Suzuki, S. C. Silverstein, and J. El Khoury Protection from Lethal Gram-positive Infection by Macrophage Scavenger Receptor-dependent Phagocytosis J. Exp. Med., January 3, 2000; 191(1): 147 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Gregory, E. J. Wing, K. L. Danowski, N. van Rooijen, K. F. Dyer, and D. J. Tweardy IL-6 Produced by Kupffer Cells Induces STAT Protein Activation in Hepatocytes Early During the Course of Systemic Listerial Infections J. Immunol., June 15, 1998; 160(12): 6056 - 6061. [Abstract] [Full Text] [PDF]
|
|
