Journal of Leukocyte Biology, Vol 47, Issue 5 440-448, Copyright © 1990 by Society for Leukocyte Biology
JOURNAL ARTICLE |
G Rothe and G Valet
Mildred-Scheel-Labor fur Krebszellforschung, Max-Planck-Institut fur Biochemie, Martinsried, Federal Republic of Germany.
Hydroethidine (HE) and 2',7'-dichlorofluorescin (DCFH) were used for the flow cytometric measurement of reactive oxygen metabolites in leukocytes. Hydroethidine and DCFH were both rapidly oxidized in a cell-free cuvette assay to ethidium bromide (EB) and 2',7'-dichlorofluorescein (DCF) by H2O2 and peroxidase, but not by H2O2 alone, while only HE was oxidized by KO2, a source of O2-. Quiescent lymphocytes, monocytes, and neutrophils spontaneously oxidized HE to EB, while DCFH was only oxidized to a low degree. Neutrophils increased 6.9-fold in EB red fluorescence and 12.5-fold in DCF green fluorescence during the respiratory burst induced by phorbol 12-myristate 13-acetate or 6.1-fold and 4.7-fold, respectively, during the respiratory burst induced by Escherichia coli bacteria. The HE or DCFH oxidation during the respiratory burst, unlike the spontaneous HE oxidation, was not inhibitable by 10 mM NaNe indicating a non-mitochondrial source of cellular oxidants during the respiratory burst such as NADPH oxidase, which produces O2-. The oxidation of DCFH, but not of HE, was decreased in stimulated neutrophils, which were simultaneously loaded with HE and DCFH. Intracellular DCFH oxidation induced by incubation of resting neutrophils with extracellular H2O2 was not influenced by the presence of HE. This indicates that HE is oxidized at an earlier step in the reactive oxygen metabolism of neutrophils than DCFH, i.e., by early oxygen metabolites like O2-, while DCFH is oxidized in part by H2O2 and phagosomal peroxidases. The differential oxidation of HE and DCFH during simultaneous cellular staining permits the analysis of up to three functionally different neutrophil populations in septic patients. This is of interest for the determination of disease-related alterations of oxygen metabolism in quiescent and stimulated leukocytes.
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|
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|
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 M.-C. Plotkowski, H. C. C. Povoa, J.-M. Zahm, G. Lizard, G. M. B. Pereira, J.-M. Tournier, and E. Puchelle Early Mitochondrial Dysfunction, Superoxide Anion Production, and DNA Degradation Are Associated with Non-Apoptotic Death of Human Airway Epithelial Cells Induced by Pseudomonas aeruginosa Exotoxin A Am. J. Respir. Cell Mol. Biol., May 1, 2002; 26(5): 617 - 626. [Abstract] [Full Text] [PDF]
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 C. Marchetti, G. Obert, A. Deffosez, P. Formstecher, and P. Marchetti Study of mitochondrial membrane potential, reactive oxygen species, DNA fragmentation and cell viability by flow cytometry in human sperm Hum. Reprod., May 1, 2002; 17(5): 1257 - 1265. [Abstract] [Full Text] [PDF]
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D. P. Pearlstein, M. H. Ali, P. T. Mungai, K. L. Hynes, B. L. Gewertz, and P. T. Schumacker Role of Mitochondrial Oxidant Generation in Endothelial Cell Responses to Hypoxia Arterioscler Thromb Vasc Biol, April 1, 2002; 22(4): 566 - 573. [Abstract] [Full Text] [PDF]
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 G. W. Kim, T. Kondo, N. Noshita, and P. H. Chan Manganese Superoxide Dismutase Deficiency Exacerbates Cerebral Infarction After Focal Cerebral Ischemia/Reperfusion in Mice: Implications for the Production and Role of Superoxide Radicals Stroke, March 1, 2002; 33(3): 809 - 815. [Abstract] [Full Text] [PDF]
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 S. Devadas, L. Zaritskaya, S. G. Rhee, L. Oberley, and M. S. Williams Discrete Generation of Superoxide and Hydrogen Peroxide by T Cell Receptor Stimulation: Selective Regulation of Mitogen-Activated Protein Kinase Activation and Fas Ligand Expression J. Exp. Med., January 7, 2002; 195(1): 59 - 70. [Abstract] [Full Text] [PDF]
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K. Dvorakova, C. M. Payne, M. E. Tome, M. M. Briehl, M. A. Vasquez, C. N. Waltmire, A. Coon, and R. T. Dorr Molecular and Cellular Characterization of Imexon-resistant RPMI8226/I Myeloma Cells Mol. Cancer Ther., January 1, 2002; 1(3): 185 - 195. [Abstract] [Full Text] [PDF]
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Z. Xu, M. V. Cohen, J. M. Downey, T. L. Vanden Hoek, and Z. Yao Attenuation of oxidant stress during reoxygenation by AMP 579 in cardiomyocytes Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2585 - H2589. [Abstract] [Full Text] [PDF]
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 G. Delogu, S. Moretti, G. Famularo, S. Marcellini, G. Santini, A. Antonucci, M. Marandola, and L. Signore Mitochondrial Perturbations and Oxidant Stress in Lymphocytes From Patients Undergoing Surgery and General Anesthesia Arch Surg, October 1, 2001; 136(10): 1190 - 1196. [Abstract] [Full Text] [PDF]
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M. M. Tarpey and I. Fridovich Methods of Detection of Vascular Reactive Species: Nitric Oxide, Superoxide, Hydrogen Peroxide, and Peroxynitrite Circ. Res., August 3, 2001; 89(3): 224 - 236. [Abstract] [Full Text] [PDF]
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 V. W. HU, D. S. HEIKKA, P. B. DIEFFENBACH, and L. HA Metabolic radiolabeling: experimental tool or Trojan horse? 35S-Methionine induces DNA fragmentation and p53-dependent ROS production FASEB J, July 1, 2001; 15(9): 1562 - 1568. [Abstract] [Full Text] [PDF]
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H. Liu, B. C. McPherson, X. Zhu, M. L. A. Da Costa, V. Jeevanandam, and Z. Yao Role of nitric oxide and protein kinase C in ACh-induced cardioprotection Am J Physiol Heart Circ Physiol, July 1, 2001; 281(1): H191 - H197. [Abstract] [Full Text] [PDF]
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 A. Summerfield, K. Zingle, S. Inumaru, and K. C. McCullough Induction of apoptosis in bone marrow neutrophil-lineage cells by classical swine fever virus J. Gen. Virol., June 1, 2001; 82(6): 1309 - 1318. [Abstract] [Full Text]
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K. Dvorakova, C. N. Waltmire, C. M. Payne, M. E. Tome, M. M. Briehl, and R. T. Dorr Induction of mitochondrial changes in myeloma cells by imexon Blood, June 1, 2001; 97(11): 3544 - 3551. [Abstract] [Full Text] [PDF]
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 M.-P. Wautier, O. Chappey, S. Corda, D. M. Stern, A. M. Schmidt, and J.-L. Wautier Activation of NADPH oxidase by AGE links oxidant stress to altered gene expression via RAGE Am J Physiol Endocrinol Metab, May 1, 2001; 280(5): E685 - E694. [Abstract] [Full Text] [PDF]
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C. Elbim, H. Reglier, M. Fay, C. Delarche, V. Andrieu, J. El Benna, and M.-A. Gougerot-Pocidalo Intracellular Pool of IL-10 Receptors in Specific Granules of Human Neutrophils: Differential Mobilization by Proinflammatory Mediators J. Immunol., April 15, 2001; 166(8): 5201 - 5207. [Abstract] [Full Text] [PDF]
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J. Baran, K. Weglarczyk, M. Mysiak, K. Guzik, M. Ernst, H.-D. Flad, and J. Pryjma Fas (CD95)-Fas Ligand Interactions Are Responsible for Monocyte Apoptosis Occurring as a Result of Phagocytosis and Killing of Staphylococcus aureus Infect. Immun., March 1, 2001; 69(3): 1287 - 1297. [Abstract] [Full Text] [PDF]
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Z. Yao, B. C. McPherson, H. Liu, Z. Shao, C. Li, Y. Qin, T. L. Vanden Hoek, L. B. Becker, and P. T. Schumacker Signal transduction of flumazenil-induced preconditioning in myocytes Am J Physiol Heart Circ Physiol, March 1, 2001; 280(3): H1249 - H1255. [Abstract] [Full Text] [PDF]
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 B. C. McPherson and Z. Yao Morphine Mimics Preconditioning via Free Radical Signals and Mitochondrial KATP Channels in Myocytes Circulation, January 16, 2001; 103(2): 290 - 295. [Abstract] [Full Text] [PDF]
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 A. Rosenspire, A. Kindzelskii, and H. Petty Pulsed DC electric fields couple to natural NAD(P)H oscillations in HT-1080 fibrosarcoma cells J. Cell Sci., January 4, 2001; 114(8): 1515 - 1520. [Abstract] [PDF]
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D. J. Samuelson, M.-B Powell, M. Lluria-Prevatt, and D. F. Romagnolo Transcriptional activation of the gp91phox NADPH oxidase subunit by TPA in HL-60 cells J. Leukoc. Biol., January 1, 2001; 69(1): 161 - 168. [Abstract] [Full Text]
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M. Noursadeghi, M. C. M. Bickerstaff, J. R. Gallimore, J. Herbert, J. Cohen, and M. B. Pepys Role of serum amyloid P component in bacterial infection: Protection of the host or protection of the pathogen PNAS, December 19, 2000; 97(26): 14584 - 14589. [Abstract] [Full Text] [PDF]
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A. D. Badley, A. A. Pilon, A. Landay, and D. H. Lynch Mechanisms of HIV-associated lymphocyte apoptosis Blood, November 1, 2000; 96(9): 2951 - 2964. [Abstract] [Full Text] [PDF]
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K. Hossain, A. A. Akhand, M. Kato, J. Du, K. Takeda, J. Wu, K. Takeuchi, W. Liu, H. Suzuki, and I. Nakashima Arsenite Induces Apoptosis of Murine T Lymphocytes Through Membrane Raft-Linked Signaling for Activation of c-Jun Amino-Terminal Kinase J. Immunol., October 15, 2000; 165(8): 4290 - 4297. [Abstract] [Full Text] [PDF]
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C. A. Gunnett, D. D. Heistad, D. J. Berg, and F. M. Faraci IL-10 deficiency increases superoxide and endothelial dysfunction during inflammation Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1555 - H1562. [Abstract] [Full Text] [PDF]
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Q. Zhang and Z. Yao Flumazenil preconditions cardiomyocytes via oxygen radicals and KATP channels Am J Physiol Heart Circ Physiol, October 1, 2000; 279(4): H1858 - H1863. [Abstract] [Full Text] [PDF]
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T. S. Hiura, N. Li, R. Kaplan, M. Horwitz, J.-C. Seagrave, and A. E. Nel The Role of a Mitochondrial Pathway in the Induction of Apoptosis by Chemicals Extracted from Diesel Exhaust Particles J. Immunol., September 1, 2000; 165(5): 2703 - 2711. [Abstract] [Full Text] [PDF]
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 N. S. Chandel and P. T. Schumacker Cellular oxygen sensing by mitochondria: old questions, new insight J Appl Physiol, May 1, 2000; 88(5): 1880 - 1889. [Abstract] [Full Text] [PDF]
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R. Iyer and B. E. Lehnert Factors Underlying the Cell Growth-related Bystander Responses to {{alpha}} Particles Cancer Res., March 1, 2000; 60(5): 1290 - 1298. [Abstract] [Full Text]
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Z. Yao, J. Tong, X. Tan, C. Li, Z. Shao, W. C. Kim, T. L. vanden Hoek, L. B. Becker, C. A. Head, and P. T. Schumacker Role of reactive oxygen species in acetylcholine-induced preconditioning in cardiomyocytes Am J Physiol Heart Circ Physiol, December 1, 1999; 277(6): H2504 - H2509. [Abstract] [Full Text] [PDF]
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J. M. ESTEVE, J. MOMPO, J. GARCIA DE LA ASUNCION, J. SASTRE, M. ASENSI, J. BOIX, J. R. VIÑA, J. VIÑA, and F. V. PALLARDÓ Oxidative damage to mitochondrial DNA and glutathione oxidation in apoptosis: studies in vivo and in vitro FASEB J, June 1, 1999; 13(9): 1055 - 1064. [Abstract] [Full Text]
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P. E. Hockberger, T. A. Skimina, V. E. Centonze, C. Lavin, S. Chu, S. Dadras, J. K. Reddy, and J. G. White Activation of flavin-containing oxidases underlies light-induced production of H2O2 in mammalian cells PNAS, May 25, 1999; 96(11): 6255 - 6260. [Abstract] [Full Text] [PDF]
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S. R. RÄISÄNEN, P. LEHENKARI, M. TASANEN, P. RAHKILA, P. L. HÄRKÖNEN, and H. K. VÄÄNÄNEN Carbonic anhydrase III protects cells from hydrogen peroxide-induced apoptosis FASEB J, March 1, 1999; 13(3): 513 - 522. [Abstract] [Full Text]
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 N. Weissmann, R. Voswinckel, T. Hardebusch, S. Rosseau, H. A. Ghofrani, R. Schermuly, W. Seeger, and F. Grimminger Evidence for a role of protein kinase C in hypoxic pulmonary vasoconstriction Am J Physiol Lung Cell Mol Physiol, January 1, 1999; 276(1): L90 - L95. [Abstract] [Full Text] [PDF]
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S. Ueda, H. Nakamura, H. Masutani, T. Sasada, S. Yonehara, A. Takabayashi, Y. Yamaoka, and J. Yodoi Redox Regulation of Caspase-3(-like) Protease Activity: Regulatory Roles of Thioredoxin and Cytochrome c J. Immunol., December 15, 1998; 161(12): 6689 - 6695. [Abstract] [Full Text] [PDF]
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A. J. Krohn, E. Preis, and J. H. M. Prehn Staurosporine-Induced Apoptosis of Cultured Rat Hippocampal Neurons Involves Caspase-1-Like Proteases as Upstream Initiators and Increased Production of Superoxide as a Main Downstream Effector J. Neurosci., October 15, 1998; 18(20): 8186 - 8197. [Abstract] [Full Text] [PDF]
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W. J. Arlein, J. D. Shearer, and M. D. Caldwell Continuity between wound macrophage and fibroblast phenotype: analysis of wound fibroblast phagocytosis Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1998; 275(4): R1041 - R1048. [Abstract] [Full Text] [PDF]
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L. Virag, A. L. Salzman, and C. Szabo Poly(ADP-Ribose) Synthetase Activation Mediates Mitochondrial Injury During Oxidant-Induced Cell Death J. Immunol., October 1, 1998; 161(7): 3753 - 3759. [Abstract] [Full Text] [PDF]
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