Accuri C6 Flow Cytometer System

This Article
Right arrow Full Text (PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feldman, G. M.
Right arrow Articles by Seed, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feldman, G. M.
Right arrow Articles by Seed, J. L.

Journal of Leukocyte Biology, Vol 47, Issue 4 344-354, Copyright © 1990 by Society for Leukocyte Biology

JOURNAL ARTICLE

Physiologic oxygen tensions limit oxidant-mediated killing of schistosome eggs by inflammatory cells and isolated granulomas

GM Feldman, AM Dannenberg and JL Seed
Departments of Immunology and Infectious Diseases, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD.

Explanted hepatic granulomas, eosinophils obtained from the peritoneal cavity of schistosome-infected mice, schistosome egg granuloma macrophages, alveolar macrophages, and activated peritoneal macrophages obtained from Listeria-infected mice were miracidicidal when cultured at 21% oxygen. This activity was markedly attenuated at physiologic oxygen concentrations (1-15%). Catalase and superoxide dismutase blocked the miracidicidal activity of inflammatory cells but did not prevent granuloma-mediated egg killing. However, the biomimetic superoxide dismutase, copper (II) [diisopropyl salicylate]2, inhibited granuloma-mediated egg killing in a dose-dependent, apparently nontoxic manner. Thioglycollate-elicited macrophages did not kill schistosome egg miracidia even when cultured in 21% oxygen, unless pretreated with lipopolysaccharide. Isolated schistosome eggs initiated an oxidative burst in macrophages, as measured by superoxide anion production. This burst was suppressed at reduced oxygen concentrations. Thus schistosome egg miracidia can be killed nonspecifically by macrophages through the release of cytotoxic reactive oxygen intermediates triggered by the egg. This activity is not supported by the oxygen concentrations found in most tissues, with the possible exception of the lung. Schistosoma mansoni eggs, injected intraveneously and lodged in the pulmonary vasculature of mice, were killed rapidly, with a half life of 3.5 days. Eggs, injected into the mesenteric veins and lodged in the liver, remained fully viable for several weeks. The data suggest that the high oxygen tension of the lung allows for the increased production of reactive oxygen intermediates (ROI) by local inflammatory cells, which in turn increases their miracidicidal efficiency. Conversely, the relatively hypoxic environment of the liver decreases ROI production by local inflammatory cells and decreases their miracidicidal efficiency.


This article has been cited by other articles:


Home page
 Hum Exp ToxicolHome page
M. El-Gohary, A.-E. Yassin, and M. A Shalabya
The effect of chronic lead exposure on the course of schistosomiasis in hamsters and the protective effect of the antioxidant preparation 'Antox'
Human and Experimental Toxicology, September 1, 2003; 22(9): 481 - 490.
[Abstract] [PDF]

Home page
 J. Immunol.Home page
A. C. La Flamme, E. A. Patton, B. Bauman, and E. J. Pearce
IL-4 Plays a Crucial Role in Regulating Oxidative Damage in the Liver During Schistosomiasis
J. Immunol., February 1, 2001; 166(3): 1903 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
J. Boyd and L. Burnett
Reactive oxygen intermediate production by oyster hemocytes exposed to hypoxia
J. Exp. Biol., January 11, 1999; 202(22): 3135 - 3143.
[Abstract] [PDF]